Recombinant Human Anti-IAV HA Antibody (CT149)

CAT#: PABL-213

Recombinant Human Antibody (CT149) is capable of binding to IAV HA, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-IAV HA mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-IAV HA mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This antibody is found to neutralize all tested group 2 and some group 1 influenza A viruses by inhibiting low pH-induced, HA-mediated membrane fusion.

Tested Data
Figure 1 Recombinant Human Anti-IAV HA Antibody (PABL-213) in SDS-PAGE Figure 2 Recombinant Human Anti-IAV HA Antibody (PABL-213) in HPLC Figure 3 Anti-IAV HA Recombinant Antibody (PABL-213) in ELISA Figure 4 Anti-IAV HA Recombinant Antibody (PABL-213) in ELISA Figure 5 Anti-IAV HA Recombinant Antibody (PABL-213) in WB Figure 6 Anti-IAV HA Recombinant Antibody (PABL-213) in WB Figure 7 Anti-IAV HA Recombinant Antibody (PABL-213) in WB Figure 8 Anti-IAV HA Recombinant Antibody (PABL-213) in DB Figure 9 Anti-IAV HA Recombinant Antibody (PABL-213) in DB

Specifications

  • Immunogen
  • Influenza a virus Hemagglutinin
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native IAV HA
  • Species Reactivity
  • IAV
  • Clone
  • CT149
  • Applications
  • Can be useful in applications such as: Western blot; Neutralization; Functional Study

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Target

  • Alternative Names
  • HA; hemagglutinin; haemagglutinin
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Breast cancer biomarkers at key points during disease progression Jessica Hill
22.Feb,23
Excellent for IAV-HA Detection
The Anti-IAV-HA Antibody has been excellent for our influenza A virus detection assays. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.
Breast cancer biomarkers at key points during disease progression David Adams
29.May,22
Reliable in Immunoassays
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for IAV-HA is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
Breast cancer biomarkers at key points during disease progression Megan Martinez
13.Aug,21
Consistent High-Quality Results
This antibody has proven to be consistent and reliable in our assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for IAV-HA have been particularly beneficial.

Q&As

  1. Is the anti-IAV HA antibody suitable for use in Western blotting?

    A: Yes, the anti-IAV HA antibody (PABL-213) is suitable for use in Western blotting. It provides specific binding to the HA protein, allowing for reliable detection in Western blot assays.

  2. What are the storage recommendations for the anti-IAV HA antibody?

    A: The recommended storage condition for the anti-IAV HA antibody (PABL-213) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

  3. Can the anti-IAV HA antibody be used in immunoprecipitation assays?

    A: Yes, the anti-IAV HA antibody (PABL-213) can be used in immunoprecipitation assays. It provides specific binding to the HA protein, enabling the successful precipitation of the target protein from complex mixtures.

  4. Is the anti-IAV HA antibody effective in ELISA applications?

    A: Yes, the anti-IAV HA antibody (PABL-213) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of the HA protein.

  5. What is the optimal dilution for using the anti-IAV HA antibody in immunofluorescence?

    A: The optimal dilution for using the anti-IAV HA antibody (PABL-213) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

  1. Park, Jae-Keun, et al. "Pre-existing immunity to influenza virus hemagglutinin stalk might drive selection for antibody-escape mutant viruses in a human challenge model." Nature medicine 26.8 (2020): 1240-1246. https://doi.org/10.1038/s41591-020-0937-x
    This study investigates the selection of an HA stalk antibody-escape mutant virus in human influenza challenge participants. The researchers focused on a well-characterized influenza A virus stock containing a mixed population of wild-type and mutant viruses with a nonsynonymous single-nucleotide polymorphism (SNP) in the HA stalk (A388V). They analyzed pre-existing anti-HA stalk antibody levels and post-challenge viral shedding in participants, revealing that higher pre-challenge anti-HA stalk antibody titers were associated with the selection of the mutant virus. The study found that the A388V mutation conferred resistance to some broadly neutralizing monoclonal antibodies (bNAbs) and was rapidly selected under immune pressure, highlighting a potential challenge for HA-stalk-targeting universal influenza vaccines.
    Creative Biolabs provided several key reagents for this research, including the broadly neutralizing monoclonal antibodies CR9114 (Cat#: PABX-119), FI6V3 (Cat#: PABL-214), and CT149 (Cat#: PABL-213). These antibodies were essential for evaluating the binding and neutralization characteristics of the mutant and wild-type viruses. The specific contributions of these antibodies were crucial for understanding the structural changes and resistance mechanisms conferred by the A388V mutation, thereby aiding in the comprehensive analysis of HA stalk antibody escape and its implications for vaccine development.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABL-213, RRID: AB_3111638)

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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